Adsorption-Driven Retention in Normal-Phase Chromatography with Ternary Mobile Phases

Retention modelling in ternary solvent-strength gradient elution reversed-phase chromatography using 30 mm columns.

An optimization strategy for ternary solvent-strength gradient elution RP chromatography is described in which a two-dimensional model of gradient time (2 levels) against ternary proportions of organic modifiers (4 levels) was constructed. From the resolution surface the optimum ratio of organic modifiers could be selected. Excellent retention time and acceptable peak width and resolution simul...

Effect of mobile phase pH on the retention of nucleotides on different stationary phases for high-performance liquid chromatography

The main aim of the present investigation was to study the retention and separation of eight nucleotides with the use of seven stationary phases in RP HPLC mode. Two octadecyl columns were used; however, aminopropyl, alkylamide, cholesterol, alkyl-phosphate, and phenyl were also studied. Special attention was paid to the mobile phase buffer pH, since it appears that this factor is very influent...

Normal Phase Chromatography: Mechanisms

Looking to the future, evolutionary development can be expected because the separating power of multidimension liquid chromatography is greatly increased over single-dimension liquid chromatography. The simultaneous improvements on both the software and the chromatographic apparatus will lead to a system capable of automatically developing analytical methods for a wide range of analytes in many...

Superheated Water Mobile Phases: Liquid Chromatography

Coupled SFE}SFC has shown itself to be a very useful technique for those samples for which it is applicable. The ability to transfer all the extract to the analytical column without manipulation increases sensitivity, reduces contamination and sample handling. The overloading of capillary columns is avoided. Now that methods for using modiRers in the extraction solvent and SFE sample injection ...

UNIFIED pH OF LIQUID CHROMATOGRAPHY MOBILE PHASES